priming cytokines Search Results


cytokine receptor  (5 PRIME)
90
5 PRIME cytokine receptor
Annotated common insertion sites found by LM-PCR/shotgun cloning of AKXD Lmo2 tumors.
Cytokine Receptor, supplied by 5 PRIME, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il2rg cytokine receptor  (5 PRIME)
90
5 PRIME il2rg cytokine receptor
Annotated common insertion sites found by LM-PCR/shotgun cloning of AKXD Lmo2 tumors.
Il2rg Cytokine Receptor, supplied by 5 PRIME, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/il2rg cytokine receptor/product/5 PRIME
Average 90 stars, based on 1 article reviews
il2rg cytokine receptor - by Bioz Stars, 2026-03
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mait cell cytokine priming assay  (Institute for Clinical Pharmacodynamics)
90
Institute for Clinical Pharmacodynamics mait cell cytokine priming assay
Annotated common insertion sites found by LM-PCR/shotgun cloning of AKXD Lmo2 tumors.
Mait Cell Cytokine Priming Assay, supplied by Institute for Clinical Pharmacodynamics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mait cell cytokine priming assay/product/Institute for Clinical Pharmacodynamics
Average 90 stars, based on 1 article reviews
mait cell cytokine priming assay - by Bioz Stars, 2026-03
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Image Search Results


Annotated common insertion sites found by LM-PCR/shotgun cloning of AKXD Lmo2 tumors.

Journal: PLoS Genetics

Article Title: Murine Leukemias with Retroviral Insertions at Lmo2 Are Predictive of the Leukemias Induced in SCID-X1 Patients Following Retroviral Gene Therapy

doi: 10.1371/journal.pgen.1000491

Figure Lengend Snippet: Annotated common insertion sites found by LM-PCR/shotgun cloning of AKXD Lmo2 tumors.

Article Snippet: , Il2rg , Cytokine receptor , 5 prime , 6.244 kb , same.

Techniques: Cloning, Transduction

Annotated common insertion sites found by LM-PCR/shotgun cloning of AKXD Lmo2 tumors.

Journal: PLoS Genetics

Article Title: Murine Leukemias with Retroviral Insertions at Lmo2 Are Predictive of the Leukemias Induced in SCID-X1 Patients Following Retroviral Gene Therapy

doi: 10.1371/journal.pgen.1000491

Figure Lengend Snippet: Annotated common insertion sites found by LM-PCR/shotgun cloning of AKXD Lmo2 tumors.

Article Snippet: , Il2rg , Cytokine receptor , 5 prime , 6.396 kb , inv.

Techniques: Cloning, Transduction

A) LM-PCR identified two independent insertions in tumor 7107, 5′ of the eight coding exons of the Il2rg gene. The two viral chromosomal junctions were independently cloned and sequenced. The insertions were 396 bp apart and should be present in the same clone if they occurred on the same chromosome. Partial sequence for clone 23-10 shows the region of identity with clone 23-1 (green). For clone 23-1, we found viral sequence 5′ of this region instead of chromosome X genomic sequence. B) Two insertions in the Irs2 gene were also isolated from the same tumor. Primers were designed for one allelic insertion for Irs2 and Il2rg and real time PCR performed on the genomic DNA. Primers for a chromosome X sequence were used as reference and show a copy number of 1 for this male mouse. As shown, each insertion comprises about 50% of total tumor genomic DNA. We ruled out polyploidy for Irs2 and Il2rg genes (see ). C) Panel shows a heat map of gene expression data using the mouse Affymetrix array. The expression of genes near high frequency insertions cloned from 7107 is shown with respect to normal thymus. Log 2 intensity scale is shown under the heat map.

Journal: PLoS Genetics

Article Title: Murine Leukemias with Retroviral Insertions at Lmo2 Are Predictive of the Leukemias Induced in SCID-X1 Patients Following Retroviral Gene Therapy

doi: 10.1371/journal.pgen.1000491

Figure Lengend Snippet: A) LM-PCR identified two independent insertions in tumor 7107, 5′ of the eight coding exons of the Il2rg gene. The two viral chromosomal junctions were independently cloned and sequenced. The insertions were 396 bp apart and should be present in the same clone if they occurred on the same chromosome. Partial sequence for clone 23-10 shows the region of identity with clone 23-1 (green). For clone 23-1, we found viral sequence 5′ of this region instead of chromosome X genomic sequence. B) Two insertions in the Irs2 gene were also isolated from the same tumor. Primers were designed for one allelic insertion for Irs2 and Il2rg and real time PCR performed on the genomic DNA. Primers for a chromosome X sequence were used as reference and show a copy number of 1 for this male mouse. As shown, each insertion comprises about 50% of total tumor genomic DNA. We ruled out polyploidy for Irs2 and Il2rg genes (see ). C) Panel shows a heat map of gene expression data using the mouse Affymetrix array. The expression of genes near high frequency insertions cloned from 7107 is shown with respect to normal thymus. Log 2 intensity scale is shown under the heat map.

Article Snippet: , Il2rg , Cytokine receptor , 5 prime , 6.396 kb , inv.

Techniques: Clone Assay, Sequencing, Isolation, Real-time Polymerase Chain Reaction, Gene Expression, Expressing